The Cary Eclipse Fluorescence Spectrophotometer from Varian (now Agilent) has the following features and capabilities.
- Selected Features: (for details, please see Fluorimeter Features)
- High Sensitivity- Limit of detection
- 1.0 picoMolar fluorescein using standard cell
- 10 picoMolar fluorescein using 40 ul microcell
- 750:1 S/N for Raman band of H2O, 350 nm excitation,excitation and emission slits 10 nm.
- Wavelength Range from 200-900nm for both excitation & emission monochromator; zero order selectable
- Wavelength Accuracy of +/-0.5nm at 541.2nm and +/- 1.0 nm from 200-900 nm
- Fast scan speed of 24,000nm/min without wavelength shifts versus slower scan speeds
- Spectral band pass - Excitation: 1.5, 2.5, 5,10,20 nm & Emission: 1.5, 2.5, 5, 10, 20 nm
- Wavelength Reproducibility of +/-0.2nm
- Lamp Pulse width at half peak height ~2 us, peak power equivalent to 75 kW
- Variable Temperature Range: -10C - 100C
- Stir plate
- Wellplate Reader Accessory - It is best to use wellplates ordered from Agilent as the accessory is already aligned for them. http://www.home.agilent.com/agilent/home.jspx?cc=US&lc=eng
- Manual Polarizer Accessory
- Temperature Probe Accessory
- High Sensitivity- Limit of detection
- Applications
- Able to capture one phosphorescence data point every 1 microsecond up to 10 seconds
- Able to capture one fluorescence data point every 12.5 milliseconds up to 999 sec.
- Able to capture one bio/chemiluminescence data point every 40 microseconds up to 10 sec
- Operation Instructions
- Interesting Links
Lab:
Contact:
This is a self-train instrument, however, a user account is required to use the instrument. Please contact elsa_robertson [at] ucsb [dot] edu (Elsa Robertson) for a user account.